Varing chemical equilibrium gives kinetic parameters

We are interested in finding the kinetic parameters of a chemical reaction. Previous methods for finding these parameters rely on the dynamic behaviour of the system. This means that the methods are time-sensitive and often rely on non-linear curve fitting. In the same manner as previous techniques, we consider the concentrations of chemicals in a reaction. However, we investigate the static behaviour of the reaction at dynamic equilibrium, or steady state. Here too, the chemical concentrations depend on the kinetic parameters of the reaction. In an open reaction, the static concentrations also depends on the rate of input of the source of reacting chemical. Controlling this input rate slides the steady state along a curve in concentration space. This curve is determined by the kinetic parameters. The plane of this curve is sufficient to find the kinetic parameters. The new method we propose uses only the steady state concentration values to determine the kinetic parameters of the reaction. These values are constant once dynamic equilibrium is achieved, and so can be read accurately. Readings can be repeated readily to reduce error. Thus this new technique is simple and could produce accurate kinetic parameter estimates. 1 . http://dx.doi.org/10.1101/000547 doi: bioRxiv preprint first posted online Nov. 16, 2013;